کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235787 | 45058 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oxidation of phenyl compounds using strongly stable immobilized-stabilized laccase from Trametes versicolor
ترجمه فارسی عنوان
اکسیداسیون ترکیبات فنیل با استفاده از لاکتوز تثبیت شده با ثبات با تثبیت کننده قوی از ترامیتس ورسیکلور
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The hydrolysis of phenolic compounds using an immobilized and highly active and stable derivative of laccase from Trametes versicolor is presented. The enzyme was immobilized on aldehyde supports. For this, the enzyme was enriched in amino groups by chemical modification of its carboxyl groups. The aminated enzyme was immobilized with a high recovered activity (over 60%). Aldehyde derivatives were more stable than soluble or aminated-soluble enzyme and the reference derivatives after incubation in different inactivating conditions (high temperatures, different pH values or presence of organic cosolvents). The most stable derivative was obtained immobilizing the chemically aminated enzyme at pH 10 on aldehyde supports with a stabilization factor approximately 280 fold after incubation at pH 7 and 55 °C. In addition, it was possible to prepare immobilized derivatives with a maximal enzyme loading of 60 mg gâ1 of support. This derivative could be reused for 10 reaction cycles with negligible lost of activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 48, Issue 8, August 2013, Pages 1174-1180
Journal: Process Biochemistry - Volume 48, Issue 8, August 2013, Pages 1174-1180
نویسندگان
Veria Addorisio, Filomena Sannino, Cesar Mateo, Jose M. Guisan,