کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10235798 45058 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Architecture and functional groups of biofilms during composting with and without inoculation
ترجمه فارسی عنوان
معماری و گروه های کاربردی فیلم های بیوفیلم در طی کمپوست با و بدون تلقیح
کلمات کلیدی
بیوفیلم، کمپوست، تلقیح، طیف سنجی همبستگی دو بعدی، برچسب زدن چندگانه فلورسنت،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Detailed knowledge of the architecture and molecular structure of biofilms during composting is important for understanding the underlying mechanisms of rapid composting with inoculation. In this study, multiple fluorescent labeling and two-dimensional Fourier transform infrared (FTIR) correlation spectroscopy were used to characterize biofilms during composting with and without inoculation with a cellulose-degrading strain, Aspergillus fumigatus F12. The results showed that inoculation with A. fumigatus F12 allowed the compost to rapidly reach the thermophilic phase. Further investigation demonstrated that the role of A. fumigatus F12 during composting was to destroy the network-like structure of cellulose and to increase the contact of other biopolymers with microorganisms, as observed in situ using multiple fluorescence labeling combined with confocal laser scanning microscopy (CLSM). Two-dimensional FTIR correlation spectroscopy supported the conclusion that the critical role of inoculation in composting was attributed to the degradation of cellulose (1420 cm−1) prior to other biopolymers. In summary, multiple fluorescent labeling and two-dimensional FTIR correlation spectroscopy can be used as a novel tool for characterizing biofilms and the critical roles of microorganisms in composting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 48, Issue 8, August 2013, Pages 1222-1226
نویسندگان
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