کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235991 | 45072 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system](/preview/png/10235991.png)
چکیده انگلیسی
Enantiopure sulfoxides can be prepared via the asymmetric oxidation of sulfides using sulfide monooxygenases. Five different organic-aqueous biphasic systems were investigated for their abilities to increase the bio-oxidation efficiency of a water-insoluble sulfide (phenyl methyl sulfide, PMS). Isooctane was found to be the most suitable organic phase solvent. In this isooctane-aqueous system, the oxidation reaction was not sensitive to pH, giving high yields over the entire range of the tested pH values (5.0-11.0). In contrast, cell concentration significantly affected the reaction rate. (S)-Phenyl methyl sulfoxide ((S)-PMSO) was formed at a higher concentration and a greater enantiomeric excess (18.3Â mM and >99.0% ee(S)) in this isooctane-aqueous biphasic system than in an aqueous single-phase system (2.17Â mM and 59.9% ee(S)). The asymmetric oxidation of 150Â mM PMS in the isooctane phase was successfully carried out in a mechanically stirred bioreactor (2.4Â L) and resulted in a 49.0% yield of (S)-PMSO with enantiomeric excess greater than 99.0%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 689-694
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 689-694
نویسندگان
Ai-Tao Li, Jian-Dong Zhang, Hui-Lei Yu, Jiang Pan, Jian-He Xu,