کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235991 | 45072 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enantiopure sulfoxides can be prepared via the asymmetric oxidation of sulfides using sulfide monooxygenases. Five different organic-aqueous biphasic systems were investigated for their abilities to increase the bio-oxidation efficiency of a water-insoluble sulfide (phenyl methyl sulfide, PMS). Isooctane was found to be the most suitable organic phase solvent. In this isooctane-aqueous system, the oxidation reaction was not sensitive to pH, giving high yields over the entire range of the tested pH values (5.0-11.0). In contrast, cell concentration significantly affected the reaction rate. (S)-Phenyl methyl sulfoxide ((S)-PMSO) was formed at a higher concentration and a greater enantiomeric excess (18.3Â mM and >99.0% ee(S)) in this isooctane-aqueous biphasic system than in an aqueous single-phase system (2.17Â mM and 59.9% ee(S)). The asymmetric oxidation of 150Â mM PMS in the isooctane phase was successfully carried out in a mechanically stirred bioreactor (2.4Â L) and resulted in a 49.0% yield of (S)-PMSO with enantiomeric excess greater than 99.0%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 689-694
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 689-694
نویسندگان
Ai-Tao Li, Jian-Dong Zhang, Hui-Lei Yu, Jiang Pan, Jian-He Xu,