کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10236057 45075 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adsorption onto alumina and stabilization of cysteine proteinases from crude extract of Solanum granuloso-leprosum fruits
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Adsorption onto alumina and stabilization of cysteine proteinases from crude extract of Solanum granuloso-leprosum fruits
چکیده انگلیسی
Proteolytic biocatalysts extracted from the ripe fruits of Solanum granuloso-leprosum, a native Uruguayan tree of the Solanaceae family, were stabilized by adsorption onto gamma-alumina supports. Conditions for preparing the biocatalyst-adsorbant system were evaluated for different crude extract (CE) protein concentrations and at different pHs and temperatures. All systems assayed reached equilibrium within 2-4 h. The best proteolytic activity (measured as direct enzymatic activity) was found to be at pH 6.0 and 5 °C. The biocatalyst-adsorbant system was more stable upon storage than the free enzyme in solution, which loses activity over time due to autolytic processes. Stability (resistance to desorption) was studied after ten cycles of incubation at different pHs and temperatures. There was a general tendency to retain higher activity (more than 60%) when the incubation conditions were the same as those used for preparing the biocatalyst-adsorbant system. The best conditions for using this system to treat industrial effluents were evaluated using milk whey from cheese-making and hemoglobin (the main constituent of wastewater from cattle slaughter-houses) as trial substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 46, Issue 2, February 2011, Pages 592-598
نویسندگان
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