کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236260 | 45143 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biogenesis and secretion of overproduced protein in recombinant strains of Escherichia coli
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Recombinant bacterial strains with the genes of proteins of interest cloned in plasmids provide an oversynthesis of these proteins, which is important for their large-scale production. However, the enhanced synthesis of protein is accompanied by changes in its biogenesis and accumulation of intermediate forms corresponding to different steps of protein posttranslational modification, as well as by a change in its localization. This review surveys the peculiarities of biogenesis and localization of secreted proteins produced by recombinant strains by the example of Escherichia coli alkaline phosphatase (PhoA). They might be interesting for researchers studying the fundamental and applied aspects of protein oversynthesis by recombinant E. coli strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 509-518
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 509-518
نویسندگان
A.O. Badyakina, M.A. Nesmeyanova,