کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236382 | 45144 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimizing medium composition for TaqI endonuclease production by recombinant Escherichia coli cells using response surface methodology
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
The effect of medium composition on the TaqI endonuclease production, by recombinant Escherichia coli cells carrying a plasmid encoding TaqI endonuclease, was investigated using response surface methodology. The concentration of glucose, di-ammonium hydrogen phosphate, potassium di-hydrogen and magnesium sulphate in media were changed according to a central composite rotatable design consisting of 29 experiments and enzyme yields were determined. The results were fitted to a second order polynomial with an R2 of 0.828. The model equation was then optimized using the Nelder-Mead simplex method to maximize enzyme yield within the experimental range studied. The optimum medium composition was found to be 6 g Lâ1 glucose, 1.5 g Lâ1 (NH4)2HPO4, 8 g Lâ1 KH2PO4, and 0.8 g Lâ1 MgSO4·7H2O. The model prediction of 179 à 106 U g DCWâ1 enzyme yield at optimum conditions was experimentally verified. This value is higher than any value obtained in the initial experiments as well as in the previously reported studies. The response surface methodology was found to be useful in improving the production of recombinant TaqI endonuclease in E. coli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 5, April 2005, Pages 1633-1639
Journal: Process Biochemistry - Volume 40, Issue 5, April 2005, Pages 1633-1639
نویسندگان
İ. Emrah Nikerel, Ebru Toksoy, Betül Kırdar, Ramazan Yıldırım,