کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10374834 | 879937 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
â¶ A biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [P(LA-co-DHC/biotin)] was synthesized and served as a model polymer, on which co-existed non-specific protein adsorption and specific interaction. â¶ Three different treatment were used to modify the polymer film to let the films had different properties. â¶ A newly developed technology, Fourier transform surface plasmon resonance (FT-SPR) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (SAv) and bovine serum albumin (BSA) on the polymer films at the same time. â¶ The hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less BSA adsorption and more SAv binding. â¶ Blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus the non-specific adsorption of BSA was the weakest, while the specific binding of SAv on the blocked film was relatively the highest.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 83, Issue 2, 1 April 2011, Pages 220-228
Journal: Colloids and Surfaces B: Biointerfaces - Volume 83, Issue 2, 1 April 2011, Pages 220-228
نویسندگان
Jizheng Wei, Lesan Yan, Xiuli Hu, Xuesi Chen, Yubin Huang, Xiabin Jing,