کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
598804 1454258 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protection of enzymes from photodegradation by entrapment within alumina
ترجمه فارسی عنوان
محافظت از آنزیم ها از تخریب نوری توسط گرفتار شدن در درون آلومینا
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• Protection of enzymes from UV-light photodegradation in all of its ranges is proposed.
• Sol-gel matrices as effective UV-light protectors of enzyme inactivation and denaturation are developed.
• Co-entrapment with sun-screening molecules improves the protection of enzymes.

Most enzymes are highly sensitive to UV-light in all of its ranges and their activity can irreversibly drop even after a short time of exposure. Here we report a solution of this problem by using sol-gel matrices as effective protectors against this route of enzyme inactivation and denaturation. The concept presented here utilizes several modes of action: First, the entrapment within the rigid ceramic sol-gel matrix, inhibits denaturation motions, and the hydration shell around the entrapped protein provides extra protection. Second, the matrix itself – alumina in this report – absorbs UV light. And third, sol-gel materials have been shown to be quite universal in their ability to entrap small molecules, and so co-entrapment with well documented sun-screening molecules (2-hydroxybenzophenone, 2,2′-dihydroxybenzophenone, and 2,2′-dihydroxy-4-methoxybenzophenone) is an additional key protective tool. Three different enzymes as models were chosen for the experiments: carbonic anhydrase, acid phosphatase and horseradish peroxidase. All showed greatly enhanced UV (regions UV-A, UV-B, and UV-C) stabilization after entrapment within the doped sol-gel alumina matrices.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 146, 1 October 2016, Pages 731–736
نویسندگان
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