کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
598788 1454258 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural studies of the formation of lipoplexes between siRNA and selected bis-imidazolium gemini surfactants
ترجمه فارسی عنوان
مطالعات ساختاری از تشکیل لیپوپلکس بین siRNA و سورفاکتانت Gemini ایمیدازولیوم BIS انتخابی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• Stable lipoplexes are formed on the basis of gemini surfactants and siRNA.
• Geometry of surfactant molecule promoted the formation of hexagonal and cubic phases.
• Low-resolution structure confirmed the siRNA radiostability to synchrotron radiation.

Dicationic (gemini) surfactants are agents that can be used for the preparation of stable complexes of nucleic acids, particularly siRNA for therapeutic purposes. In this study, we demonstrated that bis-imidazolium gemini surfactants with variable lengths of dioxyalkyl linker groups (from dioxyethyl to dioxydodecyl) and dodecyl side chains are excellent for the complexation of siRNA. All of these compounds effectively complexed siRNA in a charge ratio range (p/n) of 1.5–10. The low resolution structure of siRNA oligomers was characterised by small angle scattering of synchrotron radiation (SR-SAXS) and ab initio modelling. The structures of the formed complexes were also analysed using SR-SAXS, circular dichroism studies and electrophoretic mobility tests. The most promising agents for complexation with siRNA were the surfactants that contained dioxyethyl and dioxyhexyl spacer groups.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 146, 1 October 2016, Pages 598–606
نویسندگان
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