Exploring the binding mechanism of dihydropyrimidinones to human serum albumin: Spectroscopic and molecular modeling techniques

► Binding interaction of EMPD to HSA is dominated by a static quenching procedure. ► Drug-protein complex is stabilized with hydrophobic forces and hydrogen bonding. ► The specific binding site of EMPD is located at the subdomains IIA of HSA. ► The distance between the donor and the acceptor is estimated as 4.85 nm. ► Presence of common metal ions increases the systems binding constants.