Kinetic properties of pancreatic and intestinal sPLA2 from chicken and mammals using the monomolecular film technique

► The interfacial kinetic and binding data for the pancreatic and intestinal sPLA2 from bird and mammals. ► PC, in contrast to PE and PG, were resistant to the hydrolysis by hPLA2-IIA. ► ChPLA2-IIA was found to be able to hydrolyse all phospholipids. ► The binding of sPLA2 at the lipid-water interface is governed by the electrostatic and hydrophobic forces.