کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10377894 | 880746 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Surface enrichment of proteins at quartz/water interfaces: A neutron reflectivity study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
Neutron reflectivity (NR) was used to study the adsorption of human serum albumin and human fibrinogen on quartz. The proteins were individually and sequentially adsorbed from heavy water and heavy water/methanol mixtures at pH 4 and 7.0. The technique allows for the subnanometer resolution of the adsorbed layer thickness and gross morphology. Under the conditions of our measurements we found that fibrinogen formed a distinct layer that we interpret as a mat of the protein three layers thick whereas albumin formed only diffuse layers. The adsorption pattern of the two proteins changed radically when one protein was adsorbed on top of the other (previously adsorbed). In general our measurements indicate that the adsorbed protein layers on quartz are rather loosely bound and that these layers, incorporating as much as 80% water, extend further into the bulk fluid than might have been expected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 285, Issue 2, 15 May 2005, Pages 458-468
Journal: Journal of Colloid and Interface Science - Volume 285, Issue 2, 15 May 2005, Pages 458-468
نویسندگان
Daniel Forciniti, William A. Hamilton,