کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10410362 | 894432 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A miniaturized, fully automated, PCR-based detection system has been developed for the rapid detection of bacterial pathogens. Monolithic DNA purification/real-time PCR silicon chips were fabricated and tested for their ability to purify and detect the pathogeneic bacterium Listeria monocytogenes. Using silica-coated microstructures, nucleic acids could be selectively bound, washed and eluted for subsequent real-time PCR. These microstructures were included in an integrated detection microchip containing two distinct regions, one for DNA purification and one for real-time PCR. Using an automated detection platform with integrated microprocessor, pumps, valves, thermocycler and fluorescence detection modules, microchips were used to purify and detect bacterial DNA by real-time PCR amplification using SYBR Green fluorescent dye. Between 104 and 107L. monocytogenes cells could be detected using this system with an average turnaround time of 45Â min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 107, Issue 1, 27 May 2005, Pages 332-341
Journal: Sensors and Actuators B: Chemical - Volume 107, Issue 1, 27 May 2005, Pages 332-341
نویسندگان
Nathaniel C. Cady, Scott Stelick, Madanagopal V. Kunnavakkam, Carl A. Batt,