Non-viral magnetic engineering of endothelial cells with microRNA and plasmid-DNA-An optimized targeting approach

We show that polyethyleneimine/magnetic nanoparticles based delivery vector (PEI/MNP) is suitable for production of miRNA-modified endothelial cells in terms of efficiency and safety. The obtained transfected cells can be guided to the site of interest using a magnetic field and their fate can be noninvasively traced using magnetic resonance imaging. This multifunctional approach for transient cell modification bears particular interest as a basis for clinically relevant cell engineering.114