Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

One major dilemma in the extracellular vesicle (EV) field is the lack of a consensus methodology for the isolation of high amounts of pure EVs from different biological fluids. In this study, we systematically compared the most established EV purification protocol- differential ultracentrifugation (UC) with an alternative method- ultrafiltration with size-exclusion liquid chromatography (UF-LC). Using a range of different molecular and biophysical analyses, our data indicates that UF-LC allows for the purification of higher yields of biophysically intact EVs, which is not seen with UC.102