Detection of parathyroid hormone-like hormone in cancer cell cultures by gold nanoparticle-based lateral flow immunoassays

In the context of increasing relevance of parathyroid like hormone (PTHLH) in cancer, we here present a novel lateral flow immunoassay approach based on gold nanoparticles conjugated to antibodies for the rapid and simple determination of this protein production and secretion in three human cell lines: two neuroblastoma cell lines (SK-N-AS and LA-N-1) and immortalized keratinocytes (HaCaT). The proposed method allows the quantification of PTHLH in cell lysates as well as in cell culture media.75