S-nitrosocaptopril nanoparticles as nitric oxide-liberating and transnitrosylating anti-infective technology

Previously, we demonstrated increased antimicrobial activity against E. coli with nitric oxide releasing nanoparticles (NO-np) + GSH, versus NO-np alone, due to formation of S-nitrosoglutathione (GSNO), a potent transnitrosylating agent. However, a suspension of NO-np + GSH would not be practical. In the current study, we incorporated captopril into a novel nanoparticle which can generate NO as well as S-nitrosocaptopril (SNO-CAP). In addition to sustained release of NO, SNO-CAP-np demonstrated sustained GSNO production in the presence of GSH and killed both E. coli and MRSA in a dose-dependent fashion, with a pronounced effect against E. coli.236