کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10446 684 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing
چکیده انگلیسی

We have previously introduced the use of the biomaterial chitosan to form chitosan/siRNA nanoparticles for gene silencing protocols. This present study shows that the physicochemical properties (size, zeta potential, morphology and complex stability) and in vitro gene silencing of chitosan/siRNA nanoparticles are strongly dependent on chitosan molecular weight (Mw) and degree of deacetylation (DD). High Mw and DD chitosan resulted in the formation of discrete stable nanoparticles ∼200 nm in size. Chitosan/siRNA formulations (N:P 50) prepared with low Mw (∼10 kDa) showed almost no knockdown of endogenous enhanced green fluorescent protein (EGFP) in H1299 human lung carcinoma cells, whereas those prepared from higher Mw (64.8–170 kDa) and DD (∼80%) showed greater gene silencing ranging between 45% and 65%. The highest gene silencing efficiency (80%) was achieved using chitosan/siRNA nanoparticles at N:P 150 using higher Mw (114 and 170 kDa) and DD (84%) that correlated with formation of stable nanoparticles of ∼200 nm. In conclusion, this work confirms the application of chitosan as a non-viral carrier for siRNA and the importance of polymeric properties for the optimisation of gene silencing using chitosan/siRNA nanoparticles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 6, February 2007, Pages 1280–1288
نویسندگان
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