کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532583 961630 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of lactate in synovia by microchip with contactless conductivity detection
ترجمه فارسی عنوان
کوانتیزاسیون لاکتات در سینوویا با استفاده از میکروچیپ با تشخیص هدایت بدون تماس
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This article describes the determination of lactate in synovia by microchip capillary electrophoresis (MCE) integrated with contactless conductivity detection (CCD). The optimal running buffer consists of 10 mM tris(hydroxymethyl)aminomethane, 1 mM HCl, and 0.1 mM hexadecyltrimethylammonium bromide (pH 9.1). The quantitative measurement of lactate in dilute synovia samples can be finished in less than 40 s. The results indicated that the peak area had a good linear relationship with lactate concentration in the range of 20 to 1000 μM, and the correlation coefficient was 0.9984. The average recovery was from 96.6% to 106.1%, and the interday relative standard deviation was less than 4.0% (n = 6). The limit of detection (signal/noise = 3) reached 6.5 μM. To validate the assay results, we compared the current method with the high-performance liquid chromatography method by measuring lactate in synovia samples. The data analysis verified that there was no significant difference between the two methods. Due to significant features such as low cost, integration, and miniaturization, the MCE-CCD method may have great potential in clinical diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 434, Issue 1, 1 March 2013, Pages 73-77
نویسندگان
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