کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533663 961889 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization and hybridization of oligonucleotides on maleimido-terminated self-assembled monolayers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Immobilization and hybridization of oligonucleotides on maleimido-terminated self-assembled monolayers
چکیده انگلیسی
Maleimido-terminated self-assembled monolayers were prepared via a one-step reaction of a maleimido-functionalized trichlorosilane with an oxide-covered surface. Through conjugate addition, thiol-tagged DNA was immobilized on these maleimido-functionalized self-assembled monolayers, and these immobilized oligonucleotides were further hybridized with complementary strands. The surface concentrations of oligonucleotides were quantified with radioactivity measurements. The surface concentrations of immobilized oligonucleotides increase in a nonlinear manner with increasing molar fraction of maleimido moieties on the surface and achieve high values of 1.6 (±0.6) × 1013 oligonucleotides/cm2 if monolayers having full coverage of maleimido groups are used. High hybridization efficiencies of complementary strands (∼75%) are obtained even if the surface concentration of immobilized strands exceeds a value of 5.0 × 1012 oligonucleotides/cm2. Furthermore, five cycles of denaturation and rehybridization result in only a 4% loss of the immobilized DNA after each run.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 344, Issue 2, 15 September 2005, Pages 216-223
نویسندگان
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