کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533812 | 961894 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe](/preview/png/10533812.png)
چکیده انگلیسی
A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-time-based imaging of glucose oxidase. Four methods for imaging the activity of GOx were compared, and rapid lifetime determination imaging was found to be the best in giving a linear range from 0.32 to 2.7 mUnit/mL. The detection limit is 0.32 mUnit/mL (1.7 ng mLâ1) which is similar to that of the time-resolved (gated) imaging using a microtiterplate reader. Fluorescent imaging of the activity of GOx is considered to be a useful tool for GOx-based immunoassays with potential for high-throughput screening, immobilization studies, and biosensor array technologies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 340, Issue 1, 1 May 2005, Pages 66-73
Journal: Analytical Biochemistry - Volume 340, Issue 1, 1 May 2005, Pages 66-73
نویسندگان
Meng Wu, Zhihong Lin, Michael Schäferling, Axel Dürkop, Otto S. Wolfbeis,