کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10534080 | 961904 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimizing the connectivity in disulfide-rich peptides: α-conotoxin SII as a case study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Optimizing the connectivity in disulfide-rich peptides: α-conotoxin SII as a case study Optimizing the connectivity in disulfide-rich peptides: α-conotoxin SII as a case study](/preview/png/10534080.png)
چکیده انگلیسی
We describe a strategy for the efficient, unambiguous assignment of disulfide connectivities in α-conotoxin SII, of which â¼30% of its mass is cysteine, as an example of a generalizable technique for investigation of cysteine-rich peptides. α-Conotoxin SII was shown to possess 3-8, 2-18, and 4-14 disulfide bond connectivity. Sequential disulfide bond connectivity analysis was performed by partial reduction with Tris(2-carboxyethyl)phosphine and real-time mass monitoring by direct-infusion electrospray mass spectrometry (ESMS). This method achieved high yields of the differentially reduced disulfide bonded intermediates and economic use of reduced peptide. Intermediates were alkylated with either N-phenylmaleimide or 4-vinylpyridine. The resulting alkyl products were assigned by ESMS and their alkyl positions sequentially identified via conventional Edman degradation. The methodology described allows a more efficient, rapid, and reliable assignment of disulfide bond connectivity in synthetic and native cysteine-rich peptides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 48-61
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 48-61
نویسندگان
Jon-Paul Bingham, Natalie M. Broxton, Bruce G. Livett, John G. Down, Alun Jones, Edward G. Moczydlowski,