کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10536277 962056 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the total antioxidant capacity by using a multipumping flow system with chemiluminescent detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of the total antioxidant capacity by using a multipumping flow system with chemiluminescent detection
چکیده انگلیسی
An automated flow-based procedure for assessment of total antioxidant capacity was developed. It involved a multipumping flow system, a recent approach to flow analysis, and exploited the ability of selected compounds to inhibit the chemiluminescence reactions of luminol or lucigenin with hydrogen peroxide. The system included several discretely actuated solenoid micropumps as the only active components of the flow manifold. This enabled the reproducible insertion and efficient mixing of very low volumes of sample and reagents as well as the transportation of the sample zone toward a flow-through luminometer, where the chemiluminometric response was monitored. With luminol as the chemiluminogenic reagent, linearity of the analytical curves was noted up to 3.2 × 10−4, 1.1 × 10−3, and 8.8 × 10−8 mol L−1 for Trolox, ascorbic acid, and resveratrol, respectively. With lucigenin, linear calibration plots up to 2 × 10−5 mol L−1 of Trolox and 5.7 × 10−5 mol L−1 of ascorbic acid were obtained. As favorable analytical figures of merit, the measurement precision (RSD typically between 0.2 and 2.0%, n = 10), low operational costs, low reagent consumption, sampling rate (160 and 70 h−1), and versatility should be highlighted. The proposed system can be used in distinct analytical circumstances without requiring physical reconfiguration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 345, Issue 1, 1 October 2005, Pages 90-95
نویسندگان
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