کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536298 | 962067 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical study of the interaction between cytochrome c and DNA at a modified gold electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The direct electron transfer of surface-confined horse heart cytochrome c (Cyt c) was achieved using COOH-terminated alkanethiolate-modified gold electrode. Later DNA was immobilized on the two-layer modified electrode. The quantitative determination of DNA was explored and the interaction between cytochrome c and DNA was studied. The binding site sizes were determined to be 15 bp per Cyt c molecule with double-stranded (ds) DNA and 30 nucleotides binding one Cyt c molecule with single-stranded (ss) DNA. At the dsDNA/Cyt c/MUA/Au electrode, the rate constant of oxidation electron transfer ks, ox = 1.59 Ã 10â3 cm sâ1 was obtained, at the ssDNA/Cyt c/MUA/Au electrode, the value was 2.43 Ã 10â3 m sâ1 when the scan rate was 1.0 V/s. The different electrodes were characterized with electrochemical quartz crystal microbalance and atomic force microscope.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 339, Issue 1, 1 April 2005, Pages 46-53
Journal: Analytical Biochemistry - Volume 339, Issue 1, 1 April 2005, Pages 46-53
نویسندگان
XiaoQin Ding, Jinhua Li, Jingbo Hu, Qilong Li,