Immunoprecipitation coupled with HPLC-MS/MS to discover the aromatase ligands from Glycyrrhiza uralensis

► IP coupled with LC-MS was applied to find aromatase ligands from Glycyrrhiza uralensis. ► Twenty-one compounds were found to have good affinity to aromatase. ► Seven compounds were found binding to aromatase in MCF-7 cells. ► Compounds bound to aromatase showed good aromatase inhibitory activity.