Identification of active compounds from Aurantii Immatri Pericarpium attenuating brain injury in a rat model of ischemia-reperfusion

► Activity guided screening system was developed for ischemic diseases using a human cell line. ► The system was applied to determine constituents from Aurantii Immatri Pericarpium, attenuating ischemic stroke. ► Nobiletin and tangeretin are active compounds improving cell survival under hypoxic condition. ► Intake of Aurantii Immatri Pericarpium might be developed as a functional food.