Preparative isolation and purification of xanthohumol from hops (Humulus lupulus L.) by high-speed counter-current chromatography

► Xanthohumol was efficiently purified by high-speed counter-current chromatography. ► The structure of xanthohumol was identified by UV, 1H NMR and 13C NMR. ► The prepared xanthohumol from hops extract can be used as the reference reagent.