Detection of irradiated frog (Limnonectes macrodon) leg bones by multifrequency EPR spectroscopy

► Gamma ray sterilization of foodstuff induces free radicals whose presence is evidence by EPR spectroscopy. ► X and Q bands EPR spectroscopy in combination with thermal treatments allow a better identification of free radicals. ► Experimentally determined activation energy and time stability of induced free radicals. ► Significantly thermolabile free radicals with respect to mammal bone tissue.