Anticholinesterase potential of flavonols from paper mulberry (Broussonetia papyrifera) and their kinetic studies

► The potent cholinesterase inhibition was observed on Broussonetia papyrifera. ► A prenyl group in flavonol played a critical role for cholinesterase inhibitor. ► The potent inhibitors 2-4 were showed time-dependent reversible behaviour. ► Protein affinities of inhibitors were accorded their inhibitory potencies.