Characterisation of trypsin purified from the viscera of Tunisian barbel (Barbus callensis) and its application for recovery of carotenoproteins from shrimp wastes

► Purification of trypsin from the viscera of barbel (Barbuds callensis). ► The molecular weight of the purified trypsin was estimated to be 24 kDa by SDS-PAGE. ► The optimum pH and temperature for trypsin activity were around 10.0 and 55 °C, respectively. ► The N-terminal amino acid sequence of the first 12 amino acids of trypsin was IVGGYECTPYSQ. ► The effect of B. callensis trypsin on the recovery and characteristics of carotenoprotein from shrimp shells was investigated.