Exploiting second-order advantage using PARAFAC2 for fast HPLC-DAD quantification of mixture of aflatoxins in pistachio nuts

► PARAFAC2 models in a fast analytical process for LC-DAD analysis of aflatoxins. ► Using a mixture calibration design as a calibration set. ► Examination of synthetic and real contaminated samples as validation set. ► Satisfactory quantitative and qualitative results by correct determination of factors.