24 kDa Trypsin: A predominant protease purified from the viscera of hybrid catfish (Clarias macrocephalus × Clarias gariepinus)

► Novel trypsin from hybrid catfish viscera was purified and characterised. ► The purified enzyme was stable to heat treatment up to 50 °C and over a wide pH range of 6.0-11.0. ► It was also high salt stable which can be used in high salt product.