Trypsin from zebra blenny (Salaria basilisca) viscera: Purification, characterisation and potential application as a detergent additive

► Purification of an alkaline trypsin from the viscera of zebra blenny (Salaria basilisca). ► The molecular weight of the purified trypsin was estimated to be 27 kDa by SDS-PAGE. ► The optimum pH and temperature for trypsin activity were around 9.5 and 60 °C, respectively. ► The enzyme was extremely stable in the pH range of 7.0-12.0 and highly stable at low temperatures. ► The N-terminal amino acid sequence of the first 12 amino acids of trypsin was IVGGRECTEPSQ.