Purification, structural characterization, and technological properties of an aspartyl proteinase from submerged cultures of Mucor mucedo DSM 809

► The characteristics of proteinase from Mucor mucedo were described for the first time. ► The enzyme exhibits remarkable sensitivity to thermal treatment. ► The enzyme possesses higher milk-clotting-to-total-proteolysis ratio. ► The enzyme can be considered as a potential industrial rennet candidate.