Purification and biochemical properties of a fibrinolytic enzyme from Bacillus subtilis-fermented red bean

► Bacillus subtilis fermented red bean with fibrinolytic activity was prepared. ► The purified fibrinolytic enzyme was a subtilisin-like serine protease with 29.93 kDa. ► This enzyme showed amidolytic activity for the hydrolysis of several synthetic substrates.