An enhanced preparation and purification of the major antioxidants baicalein and wogonin from Scutellariae radix

► Endogenous baicalinase-catalysed hydrolysis (EBCH) is a novel preparation method. ► Cavitation could improve the hydrolysis efficiency by producing high energy release. ► A vacuum of −0.07 MPa is the best for maximising the hydrolysis process. ► Low-pressure preparative chromatography (LPPC) is an efficient separation technology. ► High yield and purity of final products was obtained by combined EBCH and LPPC.