Analysis of coenzyme Q10 in bee pollen using online cleanup by accelerated solvent extraction and high performance liquid chromatography

► A method was developed to determine CoQ10 in bee pollen. ► The samples were extracted and purified by online cleanup of ASE. ► The method proved to be efficient, fast and environmentally friendly. ► The method was used to determine CoQ10 content in some samples. ► There was a large variation in CoQ10 content amongst the samples.