Selective determination of trace 17β-estradiol in dairy and meat samples by molecularly imprinted solid-phase extraction and HPLC

► Off-line MISPE and HPLC-UV detected trace E2 in dairy and meat samples accurately. ► The high clean-up efficiency of MISPE increased sensitivity and accuracy of HPLC. ► MISPE have better HPLC separation and higher recoveries than C18 SPE. ► MISPE and HPLC-UV can be used for routine analysis of E2 in food samples.