Preparation of Z-l-Phe-OH-NBD imprinted microchannel and its molecular recognition study

An integrated microchip was presented for selective recognition of Z-l-Phe-OH-NBD, using molecular imprinting technique. Molecularly imprinted polymer (MIP) were prepared by copolymerization in the presence of template molecule Z-l-Phe-OH-NBD, in which methacrylic acid and 4-vinylpyridine were used as functional monomers and ethylene dimethacrylate used as crosslinker. Imprinted polymer particles were introduced into a microchannel fabricated with a new material i.e. poly(methylvinylsiloxane) by simply rapid prototyping method. Imprinted effects were evaluated by laser-induced fluorescence (LIF) detection where the results indicated that good selective recognition for Z-l-Phe-OH-NBD in the imprinted polymer was obtained; the adsorption percentage of Z-l-Phe-OH-NBD was 61%. In contrast to conventional molecular imprinting analysis, integration shortened overall analysis time from 4 h to 10 min.