کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10591464 981754 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
چکیده انگلیسی
Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 23, Issue 14, 15 July 2013, Pages 4048-4051
نویسندگان
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