کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10591768 | 981761 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic study of the binding of triplex-forming oligonucleotides containing partial cationic modifications to double-stranded DNA
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
Several triplex-forming oligonucleotides (TFOs) partially modified with 2â²-O-(2-aminoethyl)- or 2â²-O-(2-guanidinoethyl)-nucleotides were synthesized and their association rate constants (kon) with double-stranded DNA were estimated by UV spectrophotometry. Introduction of cationic modifications in the 5â²-region of the TFOs significantly increased the kon values compared to that of natural TFO, while no enhancement in the rate of triplex DNA formation was observed when the modifications were in the middle and at the 3â²-region. The kon value of a TFO with three adjacent cationic modifications at the 5â²-region was found to be 3.4 times larger than that of a natural one. These results provide useful information for overcoming the inherent sluggishness of triplex DNA formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 24, Issue 14, 15 July 2014, Pages 3046-3049
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 24, Issue 14, 15 July 2014, Pages 3046-3049
نویسندگان
Yoshiyuki Hari, Shin Ijitsu, Masaaki Akabane-Nakata, Takuya Yoshida, Satoshi Obika,