کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10592785 981798 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
pH-rate profiles of l-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in l-xylulose production
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
pH-rate profiles of l-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in l-xylulose production
چکیده انگلیسی
l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD+, catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25 °C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200 min−1 and 290 mM−1 min−1, respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 24, Issue 1, 1 January 2014, Pages 173-176
نویسندگان
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