کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10593991 981818 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Clonal Rett Syndrome cell lines to test compounds for activation of wild-type MeCP2 expression
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Clonal Rett Syndrome cell lines to test compounds for activation of wild-type MeCP2 expression
چکیده انگلیسی
Rett Syndrome is an X-linked progressive neurological disorder caused by inactivation of one allele of the MECP2 gene. There are no curative treatments, and activation of wild-type MECP2 expression is one strategy for stabilizing or reversing the disease. We isolated fibroblast clones that express exclusively either the wild-type or a 32-bp-deletion mutant form of MECP2. We developed a sensitive assay for measuring wild-type MECP2 mRNA levels and tested small molecule epigenetic activators for their ability to activate gene expression. Although our pilot screen did not identify activators of MECP2 expression, it established the value of using clonal cells and defined challenges that must be overcome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 21, Issue 18, 15 September 2011, Pages 5202-5205
نویسندگان
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