کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10598832 982042 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein microarray using α-amino acids as metal tags on chips
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Protein microarray using α-amino acids as metal tags on chips
چکیده انگلیسی
Procedures for synthesizing α-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CLpro) as a model for attachment to chips containing d-/l-Glu, Asp, Orn, Ser via different transitional metal ions, it was found that the Orn chip was the best of affinity binding and stability by which Zn2+ was the best metal ion for affinity while Co2+ was the best metal ion for stability. Thus, this protein microarray can be alternatively used as a high throughput screening method for rapid detection against SARS CoV 3CLpro and/or efficient purification of other Tagged proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 15, Issue 5, 1 March 2005, Pages 1413-1416
نویسندگان
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