کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10614613 | 987167 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Attachment and proliferation of human osteoblast-like cells (MG-63) on laser-ablated titanium implant material
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
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چکیده انگلیسی
Demand is increasing for shortening the long (3-6Â months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and cellular levels by modification of the implant surface. One of the most promising of such surface modifications is laser ablation, as demonstrated by our previous results [46]. Commercially pure (CP4) sand-blasted, acid-etched titanium disks (Denti® System Ltd., Hungary) were irradiated with a KrF excimer laser (248Â nm, fluence 0.4Â J/cm2, FWHM 18Â ns, 2000 pulses), or with a Nd:YAG laser (532Â nm, 1.3Â J/cm2, 10Â ns, 200 pulses) then examined by SEM, AFM, and XPS. In vitro attachment (24Â h) and proliferation (72Â h) of MG-63 osteoblast cells were investigated via dimethylthiazol-diphenyl tetrazolium bromide (MTT), alamarBlue (AB) assays alkaline phosphatase quantification (ALP) and SEM. SEM and AFM revealed significant changes in morphology and roughness. XPS confirmed the presence of TiO2 on each sample; after Nd:YAG treatment a reduced state of Ti (Ti3Â +) was also observed. MTT, AB and ALP measurements detected an increase in the number of cells between the 24- and 72Â hour observations; however, laser treatment did not affect cell attachment and proliferation significantly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 7, October 2013, Pages 4251-4259
Journal: Materials Science and Engineering: C - Volume 33, Issue 7, October 2013, Pages 4251-4259
نویسندگان
Ágnes Györgyey, Krisztina Ungvári, Gabriella Kecskeméti, Judit Kopniczky, Béla Hopp, Albert Oszkó, István Pelsöczi, Zoltán Rakonczay, Katalin Nagy, Kinga Turzó,