Novel biosensing platform based on self-assembled supramolecular hydrogel

► Self-assembled supramolecular hydrogel was used as a biosensing platform. ► Horseradish peroxidase and polyaniline nanoparticles were co-immobilized. ► The entrapped horseradish peroxidase could retain its native conformation. ► The fabricated biosensor provided a fast amperometric response to hydrogen peroxide.