Fabrication of thin film TiO2 nanotube arrays on Co-28Cr-6Mo alloy by anodization

► TiO2 nanotube arrays have been prepared by anodization on Co-28Cr-6Mo alloy. ► Ti/Au/Ti trilayer and elevated temperature sputtered films are favorable for the formation of TiO2 nanotubes. ► The optimized electrolyte content for the growth of TiO2 nanotubes is obtained. ► The osteoblast cells on the nanotube surface show prominent filopodia.