AFM imaging of fenestrated liver sinusoidal endothelial cells

► We present our AFM observations on liver sinusoidal endothelial cells in chronological order of sample preparation. ► AFM data from fixed-dried-coated, over fixed-dried, fixed-wet, to the living cell are discussed. ► An outlook is presented how AFM can continue to contribute to unravel the onset of hepatic fenestrae.