Fluorogenic tagging of protein 3-nitrotyrosine with 4-(aminomethyl)benzene sulfonate in tissues: A useful alternative to Immunohistochemistry for fluorescence microscopy imaging of protein nitration

► 3-Nitrotyrosine in brain tissue is converted to fluorescent benzoxazole. ► Benzoxazole can be monitored by laser confocal microscopy. ► Fluorogenic labeling is an alternative strategy for monitoring 3-nitrotyrosin.