کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738599 | 1046719 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The lipophilic antioxidants α-tocopherol and coenzyme Q10 reduce the replicative lifespan of Saccharomyces cerevisiae
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کلمات کلیدی
YEPDPMCCoQROS - ROSα-Tocopherol - α-توکوفرولAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPOxidative stress - تنش اکسیداتیوdihydroethidium - دی هیدروتیدیمSaccharomyces cerevisiae - ساکارومایسس سرویزیهPro-oxidant - طرفدار اکسیدانreplicative lifespan - طول عمر تکثیر شدهyeast extract peptone dextrose - عصاره مخمر پپتون دگزستروزDHE - وLipid peroxidation - پراکسیداسیون لیپیدsynthetic complete - کامل مصنوعیCoenzyme Q - کوآنزیم QCoenzyme Q10 - کوآنزیم Q10Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Reactive oxygen species contribute to cellular ageing and an increased level of oxidative stress is often associated with ageing in many organisms. Supplementation of antioxidants has been advocated to decrease cellular oxidative stress and potentially extend lifespan. A genetically modified K6001 strain of Saccharomyces cerevisiae was employed to determine the effect of several antioxidants, including D-erythroascorbic acid, α-tocopherol and coenzyme Q10 on yeast cell replicative ageing. The replicative lifespan of the K6001 strain was assessed by absorbance change as cells exhibited a linear growth in glucose medium. In this study, water-soluble D-erythroascorbic acid had little effect on cell replicative lifespan. However, supplementation of the growth medium with the lipophilic antioxidants α-tocopherol increased oxidative stress and decreased cell lifespan. The use of α-tocopherol analogues revealed that the antioxidant activity and the membrane retention ability of α-tocopherol were involved in the lifespan reduction effect. Supplementation with either coenzyme Q10 alone, or in combination with α-tocopherol also led to a reduction in yeast replicative lifespan. This study highlights a potential pro-oxidant action of antioxidants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 49, Issue 2, 15 July 2010, Pages 237-244
Journal: Free Radical Biology and Medicine - Volume 49, Issue 2, 15 July 2010, Pages 237-244
نویسندگان
Yuen Ting Lam, Roland Stocker, Ian W. Dawes,